Open AccessLoop-mediated isothermal amplification (LAMP) based detection of bacteria: A Review
Author(s) -
Pooja Saharan,
Sudesh Dhingolia,
P. K. Khatri,
Joginder Singh Duhan,
Kumar Gahlawat Suresh
Publication year - 2014
Publication title -
african journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 1684-5315
DOI - 10.5897/ajb2013.13459
Subject(s) - loop mediated isothermal amplification , recombinase polymerase amplification , multiple displacement amplification , detection limit , nucleic acid , multiplex , microbiology and biotechnology , bacteria , dna , pathogen , polymerase chain reaction , biology , chemistry , dna extraction , chromatography , gene , genetics
Various diseases are caused by pathogenic bacteria and their diagnosis depends on accurate detection of pathogen from clinical samples. Several molecular methods have been developed including PCR, Real Time PCR or multiplex PCR which detects the pathogen accurately. However, every method has some limitations like low detection limit, whereas Loop-mediated isothermal amplification (LAMP) is a powerful and novel nucleic acid amplification method, which detects the DNA at very low level compared to other methods. This method amplifies very few copies of target DNA with high specificity, efficiency and rapidity under isothermal conditions by using a set of four specially designed primers and a DNA polymerase with strand displacement activity. This review presents detection of various bacteria by LAMP method and covers their detection limit in clinical specimens. Keywords: Bacteria, Loop-mediated isothermal amplification (LAMP), sensitive, rapid, simple. African Journal of Biotechnology , Vol 13(19), 1920-1928
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