Cryopreservation of embryonic axes of groundnut (Arachis hypogaea L.) by vitrification | Zendy

M. Abdulmalik M. | Zendy; S. Usman I. | Zendy; D. Olarewaju J. | Zendy; Abdulkadir A.D | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Cryopreservation of embryonic axes of groundnut (Arachis hypogaea L.) by vitrification

Author(s) -

M. Abdulmalik M.,

S. Usman I.,

D. Olarewaju J.,

Abdulkadir A.D

Publication year - 2014

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.13452

Subject(s) - vitrification , cryopreservation , arachis hypogaea , shoot , biology , embryonic stem cell , andrology , horticulture , botany , embryo , microbiology and biotechnology , medicine , genetics , gene

An efficient cryopreservation protocol was developed for groundnut embryonic axes using vitrification technique. Embryonic axes obtained from seeds of four groundnut genotypes were dehydrated in Plant Vitrification Solution (PVS2) solution for different durations (0, 1, 2, 3, 4 and 5 h) before plunged into liquid nitrogen and held for 1 h. Survival and shoot formation of cryopreserved embryonic axes were significantly influenced by the dehydration duration with embryonic axes treated for 2 h recording the highest survival (70%) and shoot formation (65%). Among the groundnut genotypes evaluated, Samnut 22 and 23 gave the highest survival (74.44 and 75.56%) and shoot formation (72.22 and 72.78%) after cryopreservation. Keywords: Cryopreservation, embryonic axes, groundnut, vitrification African Journal of Biotechnology , Vol. 13(2), pp. 280-285, 8 January, 2014

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research