Selection of inoculum size and Saccharomyces cerevisiae strain for ethanol production in simultaneous saccharification and fermentation (SSF) of sugar cane bagasse | Zendy

Carolina de Albuquerque Wanderley Maria | Zendy; Lucena Soares Mariana | Zendy; Ribeiro Gouveia Ester | Zendy

Open Access

Selection of inoculum size and Saccharomyces cerevisiae strain for ethanol production in simultaneous saccharification and fermentation (SSF) of sugar cane bagasse

Author(s) -

Carolina de Albuquerque Wanderley Maria,

Lucena Soares Mariana,

Ribeiro Gouveia Ester

Publication year - 2014

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.13179

Subject(s) - bagasse , ethanol fuel , fermentation , food science , sugar , chemistry , ethanol , cane , saccharomyces cerevisiae , ethanol fermentation , cellulose , yeast , reducing sugar , hydrolysis , microbiology and biotechnology , biochemistry , biology

The aim of this work was to select an inoculum concentration and a Saccharomyces cerevisiae strain for ethanol production in the simultaneous saccharification and fermentation (SSF) of sugar cane bagasse. Three concentrations of inoculum (0.4, 4.0 and 8.0 g/L) and two strains of S. cerevisiae (UFPEDA 1238 and UFPEDA 1334) were used to ferment a culture medium containing glucose as the carbon source (100 g/L). Ethanol production was lower with 0.4 g/L inoculum, independent of the strain used. Experiments with 4.0 and 8.0 g/L inoculum showed no growth and higher ethanol production. Maximum ethanol concentration was obtained with UFPEDA 1238 and 8.0 g/L inoculum concentration. These conditions were selected for ethanol production from sugar cane bagasse in SSF. Maximum ethanol concentration was attained with SSF (28 g/L), enzymatic convertibility of cellulose (76%) and volumetric productivity (0.93 g/L h). Keywords: Ethanol, Saccharomyces cerevisiae , inoculum, efficiency. African Journal of Biotechnology , Vol 13(27) 2762-2765

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