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A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia . ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia . ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based. Key words : Garcinia , genetic diversity, inter-simple sequence repeats, randomly amplified polymorphic DNA, principal component analysis.

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia