Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia | Zendy

P Utpala | Zendy; P. N O. Akishore | Zendy; Nirmal babu K. | Zendy; Kumar Senthil | Zendy; A. Parthasarathy V. | Zendy

Open Access

Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

Author(s) -

P Utpala,

P. N O. Akishore,

Nirmal babu K.,

Kumar Senthil,

A. Parthasarathy V.

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.13053

Subject(s) - rapd , biology , genetic diversity , microsatellite , dna profiling , genetics , genetic marker , garcinia , polymorphism (computer science) , genotype , dna , botany , allele , population , gene , medicine , environmental health

A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia . ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia . ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based. Key words : Garcinia , genetic diversity, inter-simple sequence repeats, randomly amplified polymorphic DNA, principal component analysis.

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