In vitro microrhizome production in Decalepis hamiltonii | Zendy

K. Thangavel | Zendy; P Ravichandran | Zendy; Gao M | Zendy; V. Manimekalai | Zendy

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In vitro microrhizome production in Decalepis hamiltonii

Author(s) -

K. Thangavel,

P Ravichandran,

Gao M,

V. Manimekalai

Publication year - 2014

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.12969

Subject(s) - callus , murashige and skoog medium , explant culture , botany , biology , horticulture , chemistry , traditional medicine , in vitro , biochemistry , medicine

Microrhizomes were produced from leaf disc derived callus of a threatened endemic medicinal plant - Decalepis hamiltonii. Murashige and Skoog (MS) medium supplemented with 2 mM 6-benzyleaminopurine (BAP) and 6 mM 1-napthaleneacetic (NAA) acid was found to be optimum for rapid callus induction and establishment from leaf disc explants. Further differentiation of callus into microrhizome was conquered in MS medium supplemented with 4 mM indole-3-butyric acid (IBA) and 8 mM NAA. A maximum of 20 microrhizomes in a cluster was produced within 90 days. Yeast extract (0.05%) and polyvinylpyrrolidone (0.05%) further enhanced the microrhizome formation when supplemented along with plant growth regulators (PGRs). Keywords: Decalepis hamiltonii , microrhizome, herbal medicine, secondary metabolites African Journal of Biotechnology , Vol 13(11), 1308-1313

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