The effects of three different grinding methods in DNA extraction of cowpea (Vigna unguiculata L. Walp) | Zendy

Tan Huaqiang | Zendy; Manman Tie | Zendy; Zhang Li | Zendy; Yanxia Zhu | Zendy; Huanxiu Li | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

The effects of three different grinding methods in DNA extraction of cowpea (Vigna unguiculata L. Walp)

Author(s) -

Tan Huaqiang,

Manman Tie,

Zhang Li,

Yanxia Zhu,

Huanxiu Li

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.12229

Subject(s) - vigna , extraction (chemistry) , grinding , liquid nitrogen , ammonium bromide , dna extraction , nitrogen , chemistry , bromide , chromatography , dna , ammonium , mortar , materials science , polymerase chain reaction , botany , metallurgy , biochemistry , biology , inorganic chemistry , composite material , organic chemistry , pulmonary surfactant , gene

Rapid DNA extraction is a prerequisite for molecular studies. Generally, plant tissue is ground in liquid nitrogen to isolate DNA; but, liquid nitrogen is dangerous and volatile. Besides, liquid nitrogen is not always available in many developing countries. To investigate if high quality DNA could be obtained for downstream PCR analysis without liquid nitrogen, the cowpea DNA was extracted by Hexadecyl trimethyl ammonium bromide cetyl trimethylammonium bromide (CTAB) method and sodium dodecyl sulphate (SDS) method, respectively, each with three different grinding methods, including ground in liquid nitrogen, in preheated mortar and in non-preheated mortar. The DNA was compared according to their yield, purity, integrity and functionality. The results showed that high quality DNA could be obtained by three grinding methods both in CTAB method and SDS method. Without liquid nitrogen, grinding plant tissue in preheated or non-preheated mortar with extraction buffer to extract DNA is feasible. Keywords : Cowpea ( Vigna unguiculata ), grinding method, liquid nitrogen, DNA extraction African Journal of Biotechnology Vol. 12(16), pp. 1946-1951

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research