Screening model for indolocarbazole-producing microorganisms by molecular probe combined with high performance liquid chromatography (HPLC) method | Zendy

Zhenping Shi | Zendy; Dong Jiaojiao | Zendy; Lin Wen-han | Zendy; Aigang Yang | Zendy; Jiuming Zhang | Zendy; Tian Li | Zendy

Open Access

Screening model for indolocarbazole-producing microorganisms by molecular probe combined with high performance liquid chromatography (HPLC) method

Author(s) -

Zhenping Shi,

Dong Jiaojiao,

Lin Wen-han,

Aigang Yang,

Jiuming Zhang,

Tian Li

Publication year - 2015

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2013.12028

Subject(s) - primer (cosmetics) , microorganism , chemistry , high performance liquid chromatography , chromatography , biochemistry , biology , bacteria , genetics , organic chemistry

Indolocarbazole (ICZ) alkaloid constitutes a group of natural products which manifested powerful biological activity, especially anticancer. However, it was laborious to find ICZs-yield organism when anticancer activity was adopted as selection target. The specificity of 6 primer pairs was detected and the results revealed that primer rebBN1/rebBC1 possessed preferable specificity because indolocarbazole-producers DNA was exclusively amplified and no target fragment was amplified from any of indolocarbazole nonproducers. Therefore, primer rebBN1/rebBC1 could specifically identify indolocarbazole-producers from microorganism strains. Key words: Indolocarbazole, rebBN1/rebBC1, ICZs-producing microorganism screening.

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