Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential | Zendy

Patil Rajashree | Zendy; S Bagde U | Zendy

Open Access

Enrichment and isolation of microbial strains degrading bioplastic polyvinyl alcohol and time course study of their degradation potential

Author(s) -

Patil Rajashree,

S Bagde U

Publication year - 2015

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2011.3980

Subject(s) - biodegradation , polyvinyl alcohol , pseudomonas , mineralization (soil science) , chemistry , microbiology and biotechnology , enrichment culture , microbial biodegradation , bacteria , degradation (telecommunications) , strain (injury) , bioplastic , food science , microbiological culture , microorganism , biology , organic chemistry , nitrogen , telecommunications , genetics , anatomy , computer science , ecology

Polyvinyl alcohol (PVA) degrading bacterial strains were isolated from various environmental sites rich in plastic wastes by using the enrichment culture technique. Among the various isolated strains, the selected potent PVA degrading bacterial strains were tentatively characterized as Bacillus and Pseudomonas sp. The time course of the PVA degradation potential of the characterized strains in growth media containing PVA as a major carbon source was evaluated using the spectropho-tometric assay method. This was done by determining the residual PVA remaining in the culture media, increase in cell growth and change in pH of the media over a period of twenty days. The ultimate biodegradation (mineralization) of PVA to its mineral constituents CO2 and H2O was determined by the CO2 evolution test. The strain characterized as Bacillus sp. showed 65% of PVA degradation as determined by spectrophotometric assay and 45.4% of mineralization of PVA over a period of 20 days. The strain characterized as Pseudomonas sp. showed 42% of PVA degradation as determined by spectrophoto-metric assay and 28.9% of mineralization of PVA over a period of 20 days. Key words: Polyvinyl alcohol (PVA) degrading bacteria, isolation, ultimate biodegradation, mineralization.

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