Open AccessAgrobacterium mediated transformation of Tunisian Cucumis melo cv. maazoun
Author(s) -
Awatef Rhimi,
Michel Hernould,
Mohamed Boussaïd
Publication year - 2007
Publication title -
african journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 1684-5315
DOI - 10.5897/ajb2007.000-2338
Subject(s) - cucumis , kanamycin , inoculation , transformation (genetics) , biology , agrobacterium , agrobacterium tumefaciens , explant culture , botany , microbiology and biotechnology , horticulture , gene , in vitro , biochemistry , antibiotics
Transgenic Cucumis melo cv. Maazoun containing the neomycin phosphotransferase II (NPT II) chimeric gene conferring resistance to kanamycin were obtained from cotyledons explants inoculated with Agrobacterium tumefaciens (GV3101) that contained the binary vector plasmid pADI. Transformed shoots were obtained on Murashige and Skoog medium supplemented with 1.50 mgl-1 IAA, 0.10mgl-1 BAP, 0.01 mgl-1 NAA and 6 mgl-1 kinetin. Transformants were selected by using only 100 mgl-1 of kanamycin and 4 days of pre-culture. Putative transformants were confirmed for transgene insertion through polymerase chain reaction (PCR) analysis. From the inoculated explants, 6.66% produced transgenic shoots.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom