Nodulation and growth response of Sesbania sesban (L.) Merr. to increasing nitrogen (ammonium) supply under glasshouse conditions

A glasshouse experiment was carried out to study the effect of ammonium-N on the nodulation, growth and N-uptake of Sesbania sesban (L.) Merr. inoculated with an effective Mesorhizobium (S. sesban ) strain. Ammonium-N was supplied twice weekly as 100 ml of nutrient solution at concentrations of 0, 100, 200, 400, 600 and 800 mg N/l. The seedlings were grown in pots with sterilized sand substrate and assessed at 5, 7 and 9 weeks after planting (WAP). Nitrogen supply significantly improved nodulation (number of nodules and nodule dry weight/plant) with treatment 100 mg N/l compared with 0 mg N/l at 5 and 7 WAP, thus indicating synergism for the N 2 -fixation symbiosis. However, with treatments ³ 200 mg N/l nodulation was either depressed or inhibited. Seedlings treated with 100-400 mg N/l generally exhibited better growth (shoot and root dry weight/plant) and N-uptake than the other treatments (0, 600 and 800 mg N/l). This study has demonstrated that S. sesban seedlings are tolerant to relatively high levels of N, and that treatment with 100 mg N/l (20 mg N per week) is necessary to stimulate an early and effective N 2 -fixing symbiosis. Key Words: Ammonium, improved fallow, Mesorhizobium , N 2 -fixation, nodulation, Sesbania sesban . African Journal of Biotechnology Vol.4(1) 2005: 57-60