A Study on the use of carrot juice in the tissue culture of Daucus carota | Zendy

Daneshwar Puchooa | Zendy; R. Ramburn | Zendy

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A Study on the use of carrot juice in the tissue culture of Daucus carota

Author(s) -

Daneshwar Puchooa,

R. Ramburn

Publication year - 2004

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2004.000-2045

Subject(s) - daucus carota , auxin , carrot juice , callus , gibberellic acid , chemistry , picloram , explant culture , acetic acid , botany , food science , in vitro , horticulture , biology , biochemistry , germination , gene

Effects of using carrot juice in the in vitro growth of Daucus carota were investigated. Callus was the only form of growth obtained after five weeks in culture. The increase in fresh weight, dry weight and moisture content of the explants with increasing concentration of carrot juice in the medium was much lower when compared to either the cytokinin benzyladenine (BA) and/or the auxin picloram. Spectrophotometric and chromatographic analyses revealed the presence of both indole-3-acetic acid (IAA) and abscissic acid (ABA) in the carrot juice, while gibberellic acid was absent. Key Words: Carrot juice; auxin; abscissic acid. African Journal of Biotechnology Vol. 3 (4), 2004: 248-252

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