Biolistic transformation of Saccharomyces cerevisiae with b-glucosidase gene from Cellulomonas biazotea | Zendy

Shoukat Parvez | Zendy; Zahid Mukhtar | Zendy; Faisal Rashid | Zendy; Rajoka M.I. | Zendy

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Biolistic transformation of Saccharomyces cerevisiae with b-glucosidase gene from Cellulomonas biazotea

Author(s) -

Shoukat Parvez,

Zahid Mukhtar,

Faisal Rashid,

Rajoka M.I.

Publication year - 2004

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2004.000-2020

Subject(s) - saccharomyces cerevisiae , transformation (genetics) , cellobiose , yeast , chemistry , gene , biochemistry , microbiology and biotechnology , biology , hydrolysis , cellulase

A β-glucosidase genomic DNA from Cellulomonas biazotea NIAB 442 was isolated and coated onto tungsten microprojectiles for direct transformation of the gene into Saccharomyces cerevisiae . Transformation of β-glucosidase into S. cerevisae conferred the ability to hydrolyse esculin and cellobiose, indicated that the gene is expressed in the bombarded yeast. Key Words: Biolistic transformation, β-glucosidase, Cellulomonas biazotea, Saccharomyces cerevisiae . African Journal of Biotechnology Vol.3(1) 2004: 112-115

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