Characterization of Trypanosoma brucei gambiense stocks isolated from humans by RAPD fingerprinting in Cte dIvoire: another evidence for multiple infections | Zendy

Bruno Oury | Zendy; Jamonneau Vincent | Zendy; T. Michel | Zendy; Truc Philippe | Zendy

Open Access

Characterization of Trypanosoma brucei gambiense stocks isolated from humans by RAPD fingerprinting in Cte dIvoire: another evidence for multiple infections

Author(s) -

Bruno Oury,

Jamonneau Vincent,

T. Michel,

Truc Philippe

Publication year - 2004

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2004.000-2017

Subject(s) - rapd , trypanosoma brucei , biology , genetic variability , genotype , virology , genetics , genetic diversity , gene , medicine , population , environmental health

Trypanosoma brucei gambiense was isolated twice from each of 23 patients in Cote d'Ivoire. Genetic characterization using RAPD (Random Primed Amplified Polymorphic DNA) showed additional variability within a given isoenzyme profile (zymodeme), confirming that this fingerprinting method has a higher discriminative power (faster molecular clock) than isoenzymes. RAPD confirmed also the evidence of multiple infections by different genotypes in the same patient despite a low genetic variability among Trypanosoma brucei gambiense stocks. The involvement of this phenomenon in treatment failure is discussed. Key Words: Human African Trypanosomiasis, Trypanosoma brucei gambiense , RAPD, multiple infections. African Journal of Biotechnology Vol.3(1) 2004: 94-98

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