Analysis of AVR4 promoter by sequential response-element deletion | Zendy

Yetunde Olukosi | Zendy; Bamidele Iwalokun | Zendy

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Analysis of AVR4 promoter by sequential response-element deletion

Author(s) -

Yetunde Olukosi,

Bamidele Iwalokun

Publication year - 2003

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2003.000-1038

Subject(s) - chloramphenicol acetyltransferase , mutant , acetyltransferase , biology , chloramphenicol , plasmid , gene , virginiamycin , transferase , biochemistry , chemistry , promoter , enzyme , antibiotics , gene expression , acetylation

Several reports have associated the variability in physico-chemical properties of avidin protein to dynamism inherent in the consensus regulatory networks within the promoter region of avidin genes. An Avr4 promoter region ligated to chloramphenicol acetyltransferase plasmid vector (pBLCAT2) to produce recombinant plasmid Avr4pBLCAT2 was sequentially deleted to produce five distinct mutants: Avr4pBLCAT2907-176, Avr4pBLCAT2809-176, Avr4pBLCAT2789-176, Avr4pBLCAT2429-176 and Avr4pBLCAT2 302-176. The transformants elicited different chloramphenicol acetyl transferase (CAT) activities. (African Journal of Biotechnology: 2003 2(7): 185-188)

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