Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] varieties determined by ARA and RAPD techniques | Zendy

Fall Laiuml ty | Zendy; Diaga Diouf | Zendy; Arama Fall-Ndiaye Mame | Zendy; Abaye Badiane Franccedil ois | Zendy; Mamadou Gueye | Zendy

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Genetic diversity in cowpea [Vigna unguiculata (L.) Walp.] varieties determined by ARA and RAPD techniques

Author(s) -

Fall Laiuml ty,

Diaga Diouf,

Arama Fall-Ndiaye Mame,

Abaye Badiane Franccedil ois,

Mamadou Gueye

Publication year - 2003

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb2003.000-1009

Subject(s) - vigna , rapd , genetic diversity , biology , germplasm , nitrogen fixation , inoculation , cultivar , horticulture , microbiology and biotechnology , botany , genetics , bacteria , population , demography , sociology

Cowpea, Vigna unguiculata (L.) Walp. presents phenotypical variabilities and in order to study the genetic diversity of cultivated Senegalese varieties, two experimental approaches were used. First, a physiological characterization based on nitrogen fixation was used to assess cowpea breeding lines. Inoculation with two Bradyrhizobium strains (NGR234 and ISRA312), showed a difference in nitrogen fixation potential between the cowpea varieties. Diongoma is the highest nitrogen fixing variety, whereas Mouride is the lowest. The second approach employed genetic characterization based on DNA polymorphism to screen. Results suggest that random amplified polymorphic DNA (RAPD) technology can be used to reorganize the national germplasm in order to eliminate the putative duplicates, and to identify elite varieties.

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