Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies | Zendy

Anita Rawat | Zendy; Barthwal Santan | Zendy; Haiming Shi | Zendy

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Genomic DNA extraction from sapwood of Pinus roxburghii for polymerase chain reaction studies

Author(s) -

Anita Rawat,

Barthwal Santan,

Haiming Shi

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb12.2733

Subject(s) - rapd , pinus roxburghii , genomic dna , polymerase chain reaction , dna extraction , dna , biology , extraction (chemistry) , microsatellite , dna profiling , microbiology and biotechnology , dna sequencing , botany , chemistry , genetics , chromatography , gene , population , genetic diversity , medicine , allele , environmental health

A method for extraction of genomic DNA from sapwood tissues of mature tall trees of Pinus roxburghii, where collection of needle tissues is extremely difficult has been standardized. The extracted DNA was comparable to that obtained from the needle tissue in terms of yield and purity. The yield of extracted DNA ranged from 6.98 to 19.668 μg / 100 mg tissue and A260 / A280 ratio ranged from 1.70 to 1.87. The polymerase chain reaction (PCR) amplification of the DNA extracted from sapwood tissue using random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR) and simple sequence repeat (SSR) markers was similar to that of DNA extracted from the needle tissues. Keywords : Pinus roxburghii, DNA extraction, sapwood, random amplification of polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), simple sequence repeat (SSR) African Journal of Biotechnology Vol. 12(15), pp. 1732-1735

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