Development of an indirect enzyme-linked immunosorbent assay (ELISA) assay based on a recombinant truncated VP2 (tVP2) protein for the detection of canine parvovirus antibodies | Zendy

Lijun Shi | Zendy; Jing Wang | Zendy; Peng Wang | Zendy; Gang Li | Zendy; Miaomiao Gong | Zendy; Weifeng Yuan | Zendy; Hongfei Zhu | Zendy

Open Access

Development of an indirect enzyme-linked immunosorbent assay (ELISA) assay based on a recombinant truncated VP2 (tVP2) protein for the detection of canine parvovirus antibodies

Author(s) -

Lijun Shi,

Jing Wang,

Peng Wang,

Gang Li,

Miaomiao Gong,

Weifeng Yuan,

Hongfei Zhu

Publication year - 2012

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb12.2446

Subject(s) - recombinant dna , canine parvovirus , antibody , microbiology and biotechnology , fusion protein , virology , detection limit , parvovirus , biology , porcine parvovirus , enzyme , plasmid , chemistry , gene , virus , biochemistry , immunology , chromatography

By removing the N-terminal hydrophobic sequence, truncated VP2 (tVP2) genes were cloned into the pET-32a (+) plasmid and subsequently expressed as His fusion proteins. The purified recombinant tVP2 proteins were specific to canine parvovirus (CPV), and one of them was used in an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of CPV antibodies. The minimum detection limit of this method was 1:1280. There was good agreement between tVP2-based indirect ELISA and the commercially available diagnostic kit. The results suggest that the recombinant tVP2 protein-based ELISA could be used to detect CPV antibodies. Key words : Canine parvovirus, recombinant truncated VP2 (tVP2), enzyme-linked immunosorbent assay (ELISA), antibody detection.

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