In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis | Zendy

J. Bello | Zendy; Iglesias | Zendy; Lourdes reu | Zendy; Laacute zaro Saacute nchez Velaacute squez | Zendy; Joseacute Casas Martiacute nez | Zendy; Nancy Santana Buzzy | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

In vitro regeneration of Pinus brutia Ten. var. eldarica (Medw.) through organogenesis

Author(s) -

J. Bello,

Iglesias,

Lourdes reu,

Laacute zaro Saacute nchez Velaacute squez,

Joseacute Casas Martiacute nez,

Nancy Santana Buzzy

Publication year - 2012

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb12.2180

Subject(s) - organogenesis , pinus brutia , scarification , explant culture , micropropagation , biology , germination , botany , shoot , horticulture , in vitro , pinus <genus> , dormancy , biochemistry , gene

This paper describes two in vitro regeneration systems through direct and indirect organogenesis in Pinus brutia using fascicles aseptic cultures as explants. Mechanical scarification and gibberellic acid (GA3) were evaluated on in vitro seed germination. Scarification was the treatment that allowed for in vitro seed germination. The highest direct organogenic response was obtained in Murashige and Skoog (MS) medium containing 4.5 μΜ thidiazuron, whereas the highest indirect organogenesis was obtained with 9.8 μΜ thidiazuron and 3.4 μΜ paclobutrazol. The isolated shoots were rooted on MS medium supplemented with 1.70 μM indoleacetic acid. A large variation in root ability was observed among plantlets. These results suggest that both regeneration systems can be applied to the micropropagation or genetic transformation of P. brutia

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research