Study of simple sequence repeat (SSR) polymorphism for biotic stress resistance in elite rice variety JGL 1798 | Zendy

Siva Kumar G | Zendy; Aruna Kumari K | Zendy; V Durga Rani | Zendy; M Sundaram R | Zendy; S. Vanisree | Zendy; Md. Jamaloddin | Zendy; G. Swathi | Zendy

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Study of simple sequence repeat (SSR) polymorphism for biotic stress resistance in elite rice variety JGL 1798

Author(s) -

Siva Kumar G,

Aruna Kumari K,

V Durga Rani,

M Sundaram R,

S. Vanisree,

Md. Jamaloddin,

G. Swathi

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb12.1109

Subject(s) - microsatellite , genetics , biology , polymorphism (computer science) , marker assisted selection , gene , genetic marker , genotype , allele

To provide ready to use markers for back ground selection in marker assisted breeding of rice, we used GPP 2 as donor parent for xa13, Xa21, Gm4 resistance to bacterial blight, gall midge and NLR 145 as another donor parent for Pi-k h gene resistance to blast and JGL 1798 as recurrent parent was investigated using 128 simple sequence repeat (SSR) primers covered on chromosome number 1-12. The results reveal that 36 HRM primers showed distinct polymorphism among the donor and recurrent parents studied indicating the robust nature of microsatellites in revealing polymorphism. Based on this study, the large range of similarity values for related cultivars using microsatellites provides greater confidence for the assessment of simple sequence repeats (SSR) polymorphism. Keywords : Simple sequence repeats (SSR), parental polymorphism, JGL 1798, hyper variable markers African Journal of Biotechnology Vol. 12(40), pp. 5833-5838

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