Overexpression of homogentisate phytyltransferase in lettuce results in increased content of vitamin E
Author(s) -
Weiwei Ren,
Lingxia Zhao,
Yuliang Wang,
Cui Lijie,
Tang Yueli,
Xiaofen Sun,
Kexuan Tang
Publication year - 2011
Publication title -
african journal of biotechnology
Language(s) - English
Resource type - Journals
ISSN - 1684-5315
DOI - 10.5897/ajb11.982
Subject(s) - lactuca , chemistry , biochemistry , agrobacterium , transgene , enzyme , gene , biology , botany
Vitamin E is a group of lipid soluble compounds, which are important antioxidants and play a crucial role in mammals and plants. They can protect the membrane from photooxidation, and they are involved in signal transduction and transcription regulation as well. The enzyme homogentisate phytyltransferase (HPT) has been demonstrated to be a key enzyme limiting tocopherol biosynthesis. In this study, HPT gene isolated from Lactuca sativa L. (LsHPT) was transferred into L. sativa (lettuce) via Agrobacterium-mediated method and 15 transgenic plants were obtained. The expression of LsHPT
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