Expression of E2F3, miR-17-5p, miR-20a and their interplay in bladder cancer

In order to explore the expression levels of E2F3, miR-17-5p, miR-20a and find out if they interact with one another in bladder cancer, 50 transitional cell carcinoma tissues, 10 normal mucosa tissues and 5637 cell line were used in this study. miR-17-5p, miR-20a, E2F3 gene and E2F3 proteinum were significantly amplifed and overexpressed in cancer tissues, and with advancing of the tumor grade, E2F3 gene and E2F3 proteinum increased. The 5637 cell showed significantly high expression of E2F3 proteinum and the proteinum could be down-regulated by miR-17-5p and miR-20a, whereas enforced overexpression of E2F3 was shown to activate transcription of miR-17-5p and miR-20a. The miR-17-5p, miR-20a, E2F3 gene and E2F3 proteinum were significantly amplified and overexpressed, and they interacted with one another in bladder cancer.   Key words: E2F3, miR-17-5p, miR-20a, bladder cancer, gene regulation.