AtCCX1 transports Na+ and K+ in Pitch pastoris | Zendy

Chen hiyuan | Zendy; Yongjun Wu | Zendy; Lijun Di | Zendy; Yufang Shen | Zendy; Wang Guodong | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

AtCCX1 transports Na+ and K+ in Pitch pastoris

Author(s) -

Chen hiyuan,

Yongjun Wu,

Lijun Di,

Yufang Shen,

Wang Guodong

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.455

Subject(s) - chemistry , vacuole , sodium , strain (injury) , vanadate , yeast , biophysics , biochemistry , biology , cytoplasm , organic chemistry , anatomy

Compartmentation of Na + into vacuoles is an important way for cells to avoid the toxic effects of salt. Here, Arabidopsis AtCCX1 was studied in Pitch pastoris GS115. Yeast expressing AtCCX1 grew better in high H + concentration medium and high salt medium than original strain and increased Na + accumulation and decreased K + accumulation. AtCCX1 was located in tonoplast in yeast. Transport essays were indicated by fluorescence SBFI and PBFI, respectively. Results show that AtCCX1 simultaneously participate in transports both of Na + and K + and the process was inhibited by H + -ATPase inhibitor vanadate. In conclusion, we suggest that AtCCX1 is an H + -dependent Na + /K + exchanger. Key words : AtCCX1, SBFI, PBFI, Na + accumulation, K + accumulation.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research