In vitro antioxidant activity and inhibitory hepatic steatosis effect on oleic acid-induced fatty liver model of consecutive extracts from Rosa davurica Pall | Zendy

Ying Wei | Zendy; Cai Muyi | Zendy; Ruizeng Gu | Zendy; Lu Jun | Zendy; Feng Lin | Zendy; Baoping Ji | Zendy

Open Access

In vitro antioxidant activity and inhibitory hepatic steatosis effect on oleic acid-induced fatty liver model of consecutive extracts from Rosa davurica Pall

Author(s) -

Ying Wei,

Cai Muyi,

Ruizeng Gu,

Lu Jun,

Feng Lin,

Baoping Ji

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.3997

Subject(s) - chemistry , quercetin , oleic acid , oxygen radical absorbance capacity , chromatography , gallic acid , ascorbic acid , antioxidant , high performance liquid chromatography , ethyl acetate , catechin , phytochemical , food science , polyphenol , biochemistry , dpph

The nutrient and phytochemical composition of Rosa davurica Pall. fruit (RDF) from China were determined, including sugar, reducing sugar, ascorbic acid, caroteniods and phenolics. RDF was successively extracted with chloroform, ethyl acetate (EA), water-saturated n-butanol, ethanol and distilled water, respectively. Among the five fractions, EA fraction revealed the highest total phenolic content and the strongest antioxidant ability in cyclic voltammograms (CV) and oxygen radical absorbance capacity (ORAC) assays. Moreover, the strongest inhibitory activity against oleic acidinduced (OA-induced) fatty liver in vitro was also the EA fraction. Further, it was separated by a semipreparative high performance liquid chromatography (HPLC) on C18 column. The most active fraction (EA-FII) of EA in all assays above, which mainly included catechin, quercetin-sedoheptulose and quercetin-94, was identified by LC/MS, HPLC and ultraviolet (UV)/V spectrum analysis. Keywords : RDF extracts, composition properties, antioxidant ability, inhibitory activity, oleic acid-induced, HPLC, LC/MS. African Journal of Biotechnology Vol. 12(31), pp. 4944-4951

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