A random amplified polymorphic DNA (RAPD) molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.) | Zendy

DU Zheng-xiang | Zendy; Hou RuiXian | Zendy; Yuying Zhu | Zendy; Li XiaoFeng | Zendy; Zhu Hongfang | Zendy; Zhenzhen Wang | Zendy

Open Access

A random amplified polymorphic DNA (RAPD) molecular marker linked to late-bolting gene in pak-choi (Brassica campestris ssp. chinensis Makino L.)

Author(s) -

DU Zheng-xiang,

Hou RuiXian,

Yuying Zhu,

Li XiaoFeng,

Zhu Hongfang,

Zhenzhen Wang

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.239

Subject(s) - rapd , bolting , bulked segregant analysis , biology , brassica , genetics , gene , genetic marker , molecular marker , botany , gene mapping , population , genetic diversity , chromosome , medicine , environmental health

Bulked segregant analysis (BSA) and random amplified polymorphic DNA (RAPD) methods were used to analyze F 2 individuals of P-27 × P-28 to screen and characterize the molecular marker linked to latebolting gene in pak-choi ( Brassica campestris ssp. chinensis Makino L.). A total of 200 random primers were used for RAPD analysis. One RAPD marker S265 750 was identified to be co-segregating with the late-bolting gene, and the genetic distances between S265 750 and late-bolting gene was 3.14 cM. The results of the study can be seen as a starting point for future researches on the pak-choi bolting gene mapping and molecular marker assisted breeding. Key words: Pak-choi, late bolting, random amplified polymorphic DNA (RAPD), bulked segregant analysis (BSA).

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