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Flowering time of tomato ( Solanum.chilense ) which is a self-incompatibility wild species flowers was determined using a series of landmark days. We established a correlation between the floral shapes and the days before anthesis. The model of tomato flower development schedule was then used to analyze the size of bud, anther, style, and the pollen scanning electron microscope (SEM) in the days before anthesis. Two new S-RNase genes of the Solanum .chilense which was the stylar SI genes were cloned. The structure of the two S-RNase was also analyzed. Finally, the different topology of the phylogenetic trees produced using available S-RNase sequences suggests that the S-RNase have different evolutionary histories. Key words: Solanum.chilense, flower development, self-incompatibility (SI), the pollen SEM

The days anthesis in relation to the floral shapes, pollen size, and S-RNase gene identifying in self-incompatibility of wild species