Optimization for ultra high pressure extraction of Scutellaria barbata by central composite design-response surface methodology

A high performance ultra high pressure (UHP) technique was developed for high yield extraction of bioactive components from Scutellaria barbata at lower temperatures, within a short time, and for less power consumption. The UHP process of scutellarin, luteolin and apigenin from S. barbata was optimized by using central composite design-response surface methodology (CCD-RSM). The optimum result of UHP was compared with those of the conventional extraction methods of circulation reflux, ultrasonic and microwave. The optimal conditions for extraction were: extracting pressure of 204.72 MPa, ethanol concentration of 61.44% and solid-liquid ratio of 1:78.79. The extraction yield of scutellarin, luteolin and apigenin from S. barbata were: 12.38, 1.76 and 0.23 mg g -1 , respectively and an integrated score of 7.18. This showed that the UHP was more effective than the conventional extraction methods. Key words: Ultra high pressure, central composite design-response surface methodology, Scutellaria barbata, high performance liquid chromatography.