Characterization of cellulase production by carbon sources in two Bacillus species | Zendy

Yan Hong | Zendy; Yingjie Dai | Zendy; Bao Xin | Zendy; Cailian Yu | Zendy; Wenting Wu | Zendy; Dexin Shan | Zendy; Liqiong Yang | Zendy; Haining Wang | Zendy; Dan Liu | Zendy; Yujie Feng | Zendy

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Characterization of cellulase production by carbon sources in two Bacillus species

Author(s) -

Yan Hong,

Yingjie Dai,

Bao Xin,

Cailian Yu,

Wenting Wu,

Dexin Shan,

Liqiong Yang,

Haining Wang,

Dan Liu,

Yujie Feng

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.1660

Subject(s) - cellulase , inducer , cellulose , monosaccharide , chemistry , food science , enzyme , bacillus (shape) , bacillales , biochemistry , microbiology and biotechnology , bacteria , biology , bacillus subtilis , genetics , gene

The induction of cellulase production in two Bacillus spp. was studied by means of measuring cellulase activities under the condition of different carbon sources. The results indicate that cellulase could not be induced by cellulose material as a sole carbon source. Instead, they could be induced by monosaccharide or disaccharide with reducing group. Moreover, the expression of cellulase components was synergistic. When cell wall/envelope enzyme and endoenzyme from two Bacillus spp. acted on these inducers, analysis of reaction products by high performance liquid chromatography (HPLC) revealed that cell wall/envelope enzyme and endoenzyme from two Bacillus spp. were inactive on these inducers. It also indicated that these inducers entered cells directly and served function of induction. Keywords: Bacillus , cellulase, induction, carbon source.

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