A simple and rapid plate assay for screening of inulin-degrading microorganisms using Lugols iodine solution | Zendy

Li Ai Xia | Zendy; Guo Li Zhong | Zendy; Qiang Fu | Zendy; Lu Dong | Zendy

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A simple and rapid plate assay for screening of inulin-degrading microorganisms using Lugols iodine solution

Author(s) -

Li Ai Xia,

Guo Li Zhong,

Qiang Fu,

Lu Dong

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.1367

Subject(s) - inulin , inulinase , microorganism , agar , hydrolysis , chemistry , chromatography , iodine , agar plate , food science , microbiology and biotechnology , biology , bacteria , biochemistry , organic chemistry , genetics

In this report, a simple and rapid agar plate assay was established for screening of halophilic, inulindegrading microorganisms. Two strains considered inulinolytic with this method were chosen and the inulinolytic activities in their culture supernatant were measured with the Somogyi-Nelson method, while their hydrolysis products of inulin were detected with TLC chromatogram. Key words: Screening, halophilic microorganism, inulinase, Lugol’s iodine solution.

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