Molecular identification of Ornithobacterium rhinotracheale in turkeys in Isfahan province of Iran | Zendy

Abbas Doosti | Zendy; Ali Sharifzadeh | Zendy; Hamed Ghasemi | Zendy; Vaez Javad | Zendy

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Molecular identification of Ornithobacterium rhinotracheale in turkeys in Isfahan province of Iran

Author(s) -

Abbas Doosti,

Ali Sharifzadeh,

Hamed Ghasemi,

Vaez Javad

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.1077

Subject(s) - polymerase chain reaction , veterinary medicine , broiler , 16s ribosomal rna , biology , microbiology and biotechnology , medicine , gene , food science , genetics

Ornithobacterium rhinotracheale (ORT ) is a gram negative, pleomorphic, rod shaped and non-motile bacterial pathogen mostly known to cause respiratory tract infections in turkeys. Ornithobacteriosis is an infectious disease of avian species that has been reported in almost all countries around the world. The objective of this study was to determine the prevalence of ORT in turkeys in Isfahan province of Iran. DNA was extracted from 375 collected tracheal swabs and lungs samples and amplified by ORT 16S rRNA gene specific primers using the PCR technique. ORT DNA was detected in 75 samples (19.93%) of broiler turkeys in Isfahan province of Iran. The results of this study demonstrated the widespread of ORT in broiler turkeys and confirmed that infection with ORT have a high prevalence in Iran.

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