High-efficiency regeneration of peanut (Arachis hypogaea L.) plants from leaf discs | Zendy

Lili Geng | Zendy; Niu Lihong | Zendy; Changlong Shu | Zendy; Fuping Song | Zendy; Dafang Huang | Zendy; Jie Zhang | Zendy

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High-efficiency regeneration of peanut (Arachis hypogaea L.) plants from leaf discs

Author(s) -

Lili Geng,

Niu Lihong,

Changlong Shu,

Fuping Song,

Dafang Huang,

Jie Zhang

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.1005

Subject(s) - arachis hypogaea , regeneration (biology) , shoot , botany , explant culture , arachis , transformation (genetics) , biology , murashige and skoog medium , chemistry , horticulture , in vitro , biochemistry , gene , microbiology and biotechnology

A high-efficiency regeneration system for peanut plants was established. The regeneration frequency of leaf discs reached 40.9% on Murashige and Skoog medium supplemented with 0.5 mg l -1 naphthylacetic acid and 0.5 mg l -1 thidiazuron. The regenerated shoots elongated, developed roots and produced seeds. This procedure was highly efficient and is feasible for the genetic transformation of peanuts. Key words : Peanut, regeneration, high efficiency.

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