The induction and growth of potato (Solanum tuberosum. L) microtubers (sante cultivar) in response to the different concentrations of 6-benzylaminopurine and sucrose | Zendy

Mostafa Ebadi | Zendy; Alireza Iranbakhsh | Zendy

Open Access

The induction and growth of potato (Solanum tuberosum. L) microtubers (sante cultivar) in response to the different concentrations of 6-benzylaminopurine and sucrose

Author(s) -

Mostafa Ebadi,

Alireza Iranbakhsh

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb11.047

Subject(s) - sucrose , gibberellic acid , solanum tuberosum , chemistry , darkness , cultivar , botany , horticulture , food science , biology , germination

The effects of different concentrations of 6-benzylaminopurine (BAP) and sucrose as induction combinations on microtuber formation and the time of this process, numbers and fresh and dry weight of microtubers were investigated. A two-stage culture was used to study the effect of hormonal and sucrose treatments. In the first stage, a liquid MS medium containing 0.5 mgl BAP + 0.4 mgl gibberellic acid (GA3) + 20 gl -1 sucrose was used for the increase of branches. The cultures of single node were grown against white light (4000 to 5000 LUX) and on sucrose for one month. In the second stage, microtuber formation induced on fluid MS medium containing different concentrations of sucrose (30, 40, 60, 80 mgl) and BAP (1, 2, 5, 10 mgl) was used in continuous darkness. Microtuber formation was investigated within 10 weeks after induction.

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