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This study was designed to establish the quality standard of Tangkening granule in order to effectively control the quality of this new Chinese traditional patent medicine in the process of production, in addition to ensure the good clinical application. Fructus gardenia, Herba gynostemmatis , Dioscorea batatas , and Polygonatum odoratum in Tangkening granule were identified by thin layer chromatography (TLC), and the content of geniposide was determined by high performance liquid chromatography (HPLC). A comprehensive validation of the method that included sensitivity, linearity, repeatability and recovery was conducted. The results show that this analytical method was simple and suitable for the original identification and quality control of this new Chinese traditional patent medicine.

african journal of biotechnology

Simultaneous qualitative and quantitative analysis of Tangkening granule using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)