Type I collagen from bullfrog (Rana catesbeiana) fallopian tube | Zendy

TzuChing Wang | Zendy; Lin Shenchung | Zendy; Shen Yingpei | Zendy; Shangyu Liu | Zendy; Wang McCall Tzuling | Zendy; Chin Menghsiung | Zendy; Lin Tachen | Zendy; Yang Clinton | Zendy; Wu Winhui | Zendy; Yang ChiChing | Zendy

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Type I collagen from bullfrog (Rana catesbeiana) fallopian tube

Author(s) -

TzuChing Wang,

Lin Shenchung,

Shen Yingpei,

Shangyu Liu,

Wang McCall Tzuling,

Chin Menghsiung,

Lin Tachen,

Yang Clinton,

Wu Winhui,

Yang ChiChing

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb10.924

Subject(s) - bullfrog , rana , absorbance , pepsin , chemistry , biochemistry , polyacrylamide gel electrophoresis , chromatography , biology , anatomy , endocrinology , enzyme

Pepsin-soluble collagen (PSC) was extracted from the fallopian tube of bullfrog ( Rana catesbeiana ) with a yield of 16.4%, on a dry weight basis. Sodium dodecyl sulphate polyacylamide-gel electrophoresis (SDS-PAGE) showed that the PSC contained two alpha components (α1 and α2) and was classified as type I collagen. PSC was found to contain 19.5% of amino acids. PSC exhibited a maximum absorbance at 230 nm, but little absorbance near to 280 nm. The denaturation temperature for PSC was determined to be 29.6°C. These results suggest that bullfrog fallopian tube has potential as a supplement to the sources of vertebrate collagens. Key words: Bullfrog (Rana catesbeiana), pepsin-soluble collagen, type I collagen.

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