Callus induction and regeneration of elite Indian maize inbreds | Zendy

A. Manivannan | Zendy; Jyoti Kaul | Zendy; Avinash Singode | Zendy; S Dass | Zendy

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Callus induction and regeneration of elite Indian maize inbreds

Author(s) -

A. Manivannan,

Jyoti Kaul,

Avinash Singode,

S Dass

Publication year - 2010

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb10.500

Subject(s) - callus , explant culture , dicamba , auxin , regeneration (biology) , biology , botany , murashige and skoog medium , horticulture , in vitro , agronomy , gene , biochemistry , weed control , microbiology and biotechnology

Five elite Indian maize inbreds namely; HKI1105, HKI1105, HKI335, CM300 and LM5 were evaluated for callus induction and regeneration. Immature embryos obtained 14 days after pollination were used as explants. Genotype, medium, type of auxin and their concentrations influenced callus induction. N6 medium supplemented with different concentration of 2,4-D (1, 2 and 3 mg/l) and Dicamba (1, 2 and 3 mg/l) were used for callus induction. N6 supplemented with 2 mg/l of 2,4-D has shown highest percentage of embryogenic callus induction. Among the five genotypes tested, CM300 gave highest percentage of embryogenic calli. CM300 and LM5 both have shown higher regeneration percentage of 12.22%. Key words: Maize, in-vitro culture and regeneration.

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