Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes | Zendy

S. A. Masudul Hoque | Zendy; Kumar Kabiraj Sanjoy | Zendy; A M Yahia Kh M | Zendy; oker | Zendy; Mondal Anupom | Zendy; M A Tareq K | Zendy

Open Access

Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes

Author(s) -

S. A. Masudul Hoque,

Kumar Kabiraj Sanjoy,

A M Yahia Kh M,

oker,

Mondal Anupom,

M A Tareq K

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb10.2519

Subject(s) - andrology , in vitro maturation , follicular fluid , pronucleus , oocyte , human fertilization , in vitro fertilisation , chemistry , medicine , biology , embryo , embryogenesis , zygote , anatomy , microbiology and biotechnology

The experiment was undertaken to study the effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation (IVM) and in vitro fertilization (IVF) of goat oocytes. COCs were collected by three techniques viz. puncture, slicing and aspiration of goat ovaries obtained at slaughterhouse. It was observed that, the total number of COCs/ovary as well as the number of abnormal COCs/ovary were significantly higher (p 0.05) on in vitro maturation and fertilization of goat oocytes as long as normal quality COCs was used. Key words : Collection techniques, cumulus oocyte complexes (COCs), in vitro maturation (IVM), in vitro fertilization (IVF).

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