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The experiment was undertaken to study the effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation (IVM) and in vitro fertilization (IVF) of goat oocytes. COCs were collected by three techniques viz. puncture, slicing and aspiration of goat ovaries obtained at slaughterhouse. It was observed that, the total number of COCs/ovary as well as the number of abnormal COCs/ovary were significantly higher (p 0.05) on in vitro maturation and fertilization of goat oocytes as long as normal quality COCs was used. Key words : Collection techniques, cumulus oocyte complexes (COCs), in vitro maturation (IVM), in vitro fertilization (IVF).

Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes