In vitro performances of hypocotyl and cotyledon explants of tomato cultivars under sodium chloride stress | Zendy

Nasher Mohamed Abdalmajid | Zendy; Mohd Razi Ismail | Zendy; Abdul Kadir Mihdzar | Zendy; Halimi Mohd Saud | Zendy

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In vitro performances of hypocotyl and cotyledon explants of tomato cultivars under sodium chloride stress

Author(s) -

Nasher Mohamed Abdalmajid,

Mohd Razi Ismail,

Abdul Kadir Mihdzar,

Halimi Mohd Saud

Publication year - 2011

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb10.2222

Subject(s) - hypocotyl , cotyledon , explant culture , cultivar , sodium , shoot , horticulture , botany , chloride , in vitro , chemistry , salt (chemistry) , biology , biochemistry , organic chemistry

A plant tissue culture technique is a good method for the evaluation and screening of plant genotypes for salt tolerance. In vitro evaluations of sodium chloride (NaCl) effects on two tomato cultivars (Pearl and Beril) were investigated with four NaCl levels (0, 25, 50 and 75 mM) using hypocotyl and cotyledon explants. The explants were cultured in MS media having 2.0 mg/l BAP along with different concentrations of NaCl. Sodium chloride stress negatively affected the growth traits and chlorophyll content. Significant differences were noticed between the cultivars followed by different NaCl levels, where the Beril responded superior than that of Pearl. The type of explant showed a difference in their response to shoots regeneration under NaCl stress, where the cotyledon explants achieved best results than hypocotyl explants. Key words: Cotyledons, hypocotyls, In vitro, salt stress, tomato.

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