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The isopentenyl transforase gene from Agrobacterium tumefaciens is one positive selectable marker genes for plant transformation. In this research, the ipt gene was placed under the control of ethanol-inducible system and was introduced into Nicotiana tabacum cv. Xanthi via Agrobacterium -mediated transformation. On a hormonefree medium (MSI) with 0.1% ethanol vapor, a large number of transgenic calli and shoots were obtained from explants that were infected with A. tumefaciens LBA4404 harboring the AlcA::ipt gene or 35S::ipt gene. On the same medium without inducer under identical culture conditions, explants can produce a larger number of calli and shoots transformed using 35S::ipt gene, but no calli or shoots were induced from explants transformed using AlcA::ipt gene. After being transferred to MSII medium, the AlcA::ipt transgenic plants displayed normal shoot morphology compared to the 35S::ipt transgenic plant lines. Our results demonstrated that ethanol-inducible ipt system can be used as a positive selection marker for tobacco plant transformation. Key words : Selectable marker, ipt gene, ethanol inducible system, transformation.

Ethanol inducible isopentenyl transferase as a high efficiency marker for tobacco transformation