Identification of RAPD markers linked to salinity tolerance in wheat | Zendy

Manzoor Bhutta Waqas | Zendy; Muhammad Hanif | Zendy

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Identification of RAPD markers linked to salinity tolerance in wheat

Author(s) -

Manzoor Bhutta Waqas,

Muhammad Hanif

Publication year - 2013

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb09.973

Subject(s) - rapd , biology , genetic diversity , salinity , primer (cosmetics) , genetic marker , bulked segregant analysis , genomic dna , population , dna profiling , molecular marker , marker assisted selection , germplasm , horticulture , botany , dna , genetics , gene , chemistry , ecology , demography , organic chemistry , sociology

-1 ) for root length at the four leaf stage. The plants were then transplanted into pots supplied with optimum water and nutrients until maturity. Genomic DNA from 50 tolerant and 50 sensitive F2 plants was extracted. The bulked segregants analysis was used in the random amplified polymorphic DNA (RAPD) technique. DNA polymorphisms were observed using 240 primers. The primer, GLE-14 amplified a 970 bp polymorphic DNA fragment and this DNA fragment can be used for marker-assisted selection to breed for salt tolerant wheat. This marker should be further used while selecting different crops under salt stress.

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