Construction and analysis of a cDNA library from yellow-fruit ginseng (Panax ginseng C.A.Meyer.) leaf tissue | Zendy

Chengjun Yang | Zendy; Jun Wang | Zendy; Chuanping Yang | Zendy; Guifeng Liu | Zendy

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Construction and analysis of a cDNA library from yellow-fruit ginseng (Panax ginseng C.A.Meyer.) leaf tissue

Author(s) -

Chengjun Yang,

Jun Wang,

Chuanping Yang,

Guifeng Liu

Publication year - 2009

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb09.630

Subject(s) - ginseng , genbank , expressed sequence tag , cdna library , biology , complementary dna , gene , gene ontology , araliaceae , accession number (library science) , traditional medicine , botany , gene expression , genetics , medicine , alternative medicine , pathology

The total RNA was isolated from yellow-fruit ginseng (Panax ginseng C.A. Meyer) leaf tissue. A cDNA library of panax ginseng leaves was constructed by using pDNR-LIB vector according to the SMART cDNA library construction kit protocol. We obtained 378 high quality sequences (GenBank accession number: ES672876-ES673253). ESTs were annotated, analyzed by BlastX and functional classified based on gene ontology, the results showed that 221 ESTs showed significant similarities to gene sequences in Nr database and were known genes, 21 ESTs were non-significance and unknown function genes, and 136 ESTs were considered novel genes. Most of the ESTs appeared to be related to physiological and cellular processes.

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