Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population | Zendy

A. Roy | Zendy; R Nassiry M | Zendy; Balal Sadeghi | Zendy; Shafagh Motlagh A | Zendy; M. Tahmoorespour | Zendy; Řeža Valizadeh | Zendy

Open Access

Implication of complex vertebral malformation and deficiency of uridine monophosphate synthase on molecular-based testing in the Iranian Holstein bulls population

Author(s) -

A. Roy,

R Nassiry M,

Balal Sadeghi,

Shafagh Motlagh A,

M. Tahmoorespour,

Řeža Valizadeh

Publication year - 2009

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb09.334

Subject(s) - holstein cattle , uridine , population , genomic dna , biology , zoology , gene , genetics , microbiology and biotechnology , rna , medicine , environmental health

The aim of this study was to identify the deficiency of uridine monophosphate synthase (DUMPS) and the complex vertebral malformation (CVM) in Iranian Holstein bulls. A total of 144 blood samples were prepared of Holstein bulls in Abbas Abad Animal Breeding Center and Ferdowsi University of Mashhad's Dairy Farm in Khorasan state of Iran. Genomic PCR-RFLP protocol was performed to amplify the polymorphic region of the bovine uridine monophosphate synthase UMPS gene. Also, genomic PCR-SSCP method was performed for CVM to amplify the polymorphic region of the bovine solute carrier family 35 member 3 (SLC35A3) genes. The results of this study demonstrated that there was no carrier of DUMPS and CVM in Iranian bulls in these centers.

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