Molecular cloning and characterization of a novel expressed sequence tag (EST) associated with fecundity in goats | Zendy

Yefeng Qiu | Zendy; Jingjing Kong | Zendy; Kesen Ma | Zendy; Xin C. | Zendy; Yi Liu | Zendy; Qiu H | Zendy

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Molecular cloning and characterization of a novel expressed sequence tag (EST) associated with fecundity in goats

Author(s) -

Yefeng Qiu,

Jingjing Kong,

Kesen Ma,

Xin C.,

Yi Liu,

Qiu H

Publication year - 2010

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb09.1715

Subject(s) - fecundity , biology , breed , cloning (programming) , gene , expressed sequence tag , complementary dna , sequence analysis , genetics , microbiology and biotechnology , population , demography , sociology , computer science , programming language

To screen the genes controlling the fecundity traits in goats, a DDRT-PCR technique was applied. We found a new EST which highly expressed in Chinese native prolific goat breed, Haimen goats. There exists a difference of EST expression level between the prolific and non-prolific goat breed, indicating EST might associate to fecundity in goats. A full-length cDNA with 2253 base pairs was obtained by the 3’- and 5’-RACE method based on the EST sequence encoding a protein segment of 201 amino acid residues. Tissue specific distribution and sequence analysis implicated the likely involvement of EST in the regulation of the hormones related to fecundity.

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