Influence of DNA treatments on Southern blot hybridization analysis of Fusarium oxysporum F. sp lycopersici and F. sp radicis-lycopersici genomic DNAs | Zendy

Samuel Balogun Olusegun | Zendy

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Influence of DNA treatments on Southern blot hybridization analysis of Fusarium oxysporum F. sp lycopersici and F. sp radicis-lycopersici genomic DNAs

Author(s) -

Samuel Balogun Olusegun

Publication year - 2008

Publication title -

african journal of biotechnology

Language(s) - English

Resource type - Journals

ISSN - 1684-5315

DOI - 10.5897/ajb08.535

Subject(s) - southern blot , fusarium oxysporum , genomic dna , biology , dna–dna hybridization , dna , digoxigenin , microbiology and biotechnology , botany , gene , in situ hybridization , genetics , gene expression

DNA samples obtained by a non-phenol/chloroform isolation method, from three races of Fusarium oxysporum f. sp. lycopersici and f. sp. radicis-lycopersici were treated in different ways with a view to evaluating the effect of three pre-electrophoresis DNA treatments on the outcome of Southern blot hybridization analysis using a Digoxigenin (DIG)-IGS fragment probe. Results showed that where DNA material is scarce, the use of undigested (native) fungal DNA not only saved time but it also gave better hybridization signal than predigestion treatments with EcoRV restriction enzyme. Hot water digestion of DNA prior electrophoresis and hybridization gave the least satisfactory result.

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