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The objective of this study is to determine the dose required to control 50% population (C50) and to reduce 50% dry matter production (GR50) of resistant biotypes of ryegrass in comparison to that of a susceptible biotype as well as to evaluate the mechanism of resistance by cyt-P450 inhibitor application. The study was conducted in a greenhouse in Rio Grande do Sul, Brazil on plants that survived clethodim herbicide application, which were suspected of possessing resistance. For plants surviving field application, the biotypes were 50% controlled with herbicide dose of 28.4and 29.5-times greater compared to that of susceptible biotypes; 50% of dry matter reduction occurred with doses of 540and 574-times greater than the susceptibility dose of a biotype, since the dose required to reduce 50% of susceptible biotype was 0.2 g a.i. ha -1 . The biotypes showed metabolism of clethodim herbicide as regards the inhibition by piperonyl butoxide, indicating that metabolism is the probable cause of control failures in the field.

Resistance characterization of Italian ryegrass (Lolium multiflorum) biotypes to clethodim herbicide