Regeneration of cotyledonary nodes from the recalcitrant melon cultivar Gacho | Zendy

Marcelo Nogueira do Amaral | Zendy; Roos Nora Fabiana | Zendy; da Silva Pinto Luciano | Zendy; Aparecida Fernando Juliana | Zendy; Antonio José | Zendy

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Regeneration of cotyledonary nodes from the recalcitrant melon cultivar Gacho

Author(s) -

Marcelo Nogueira do Amaral,

Roos Nora Fabiana,

da Silva Pinto Luciano,

Aparecida Fernando Juliana,

Antonio José

Publication year - 2014

Publication title -

african journal of agricultural research

Language(s) - English

Resource type - Journals

ISSN - 1991-637X

DOI - 10.5897/ajar2014.8584

Subject(s) - melon , cucumis , explant culture , organogenesis , shoot , biology , regeneration (biology) , cultivar , cytokinin , botany , germination , cucurbitaceae , basal shoot , horticulture , microbiology and biotechnology , in vitro , auxin , gene , biochemistry

There is lack of suitable explants for efficient shoot proliferation in recalcitrant melon cultivars restricts genetic manipulation for crop improvement. In this study, we aimed to stimulate the regeneration capability of cells of melon (Cucumis melo L.) explants in order to increase shoot proliferation efficiency during regeneration. The media of seeds germination containing different concentrations of benzylaminopurine (BAP: 0.5, 1.0, and 1.5 mg L-1) can stimulate the shoot proliferation potential of cotyledonary nodes was described. The highest number of shoots per explant was obtained when the seeds and cotyledonary nodes were cultured on Murashige and Skoog media containing 1.0 and 0.5 mg L-1 BAP, respectively, and the regeneration process occurred through organogenesis. Key words: Cucumis melo L., organogenesis, cytokinin, explants.

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