Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus | Zendy

Fathi Awad | Zendy; Ch Ch | Zendy; Ramprasath rasekaran | Zendy; Sivabalan Shanmugam | Zendy; N. Mathivanan | Zendy; Rao Aruna Prakasa | Zendy; Singaravelu Ganesan | Zendy

Open Access

Influence of hydrogen peroxide or gold nanoparticles in protoporphyrin IX mediated antimicrobial photodynamic therapy on Staphylococcus aureus

Author(s) -

Fathi Awad,

Ch Ch,

Ramprasath rasekaran,

Sivabalan Shanmugam,

N. Mathivanan,

Rao Aruna Prakasa,

Singaravelu Ganesan

Publication year - 2013

Publication title -

african journal of microbiology research

Language(s) - English

Resource type - Journals

ISSN - 1996-0808

DOI - 10.5897/2013.5885

Subject(s) - photodynamic therapy , hydrogen peroxide , staphylococcus aureus , antimicrobial , chemistry , protoporphyrin ix , photosensitizer , colloidal gold , protoporphyrin , microbiology and biotechnology , nanoparticle , bacteria , photochemistry , nanotechnology , biochemistry , materials science , organic chemistry , porphyrin , biology , genetics

Antimicrobial photodynamic therapy (APDT) has been considered to treat skin diseases infected by drug resistant microorganisms. In this work, attempts were made to study the effectiveness of APDT with protoporphyrin IX (PPIX) in the presence of gold nanoparticles (GNPs) or hydrogen peroxide (H2O2) against the human bacterial pathogen, Staphylococcus aureus. To photoactivate the PPIX, xenon light source was used at 626 nm. The bactericidal effect was analyzed by standard plate counting method. Attempts were also made to study whether fluorescence spectroscopy can be used to characterize the damage at protein level. Results of the study revealed that PPIX with H2O2 has showed higher bactericidal effect than that of PPIX alone and PPIX with GNPs. From fluorescence spectroscopic characterization it was found that protein damage is one of the reasons of bactericidal effect as there is a considerable change in the intensity of emission and fluorescence lifetime of tryptophan present in the microorganism between pre and post APDT.

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