Open AccessFour-Year Laboratory Performance of the First College of American Pathologists In Silico Next-Generation Sequencing Bioinformatics Proficiency Testing Surveys
Author(s) -
Larissa V. Furtado,
Rhona J. Souers,
Patricia Vasalos,
Jaimie G. Halley,
Dara L. Aisner,
Rakesh Nagarajan,
Karl V. Voelkerding,
Jason D. Merker,
Eric Q. Konnick
Publication year - 2022
Publication title -
archives of pathology and laboratory medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.79
H-Index - 117
eISSN - 1543-2165
pISSN - 0003-9985
DOI - 10.5858/arpa.2021-0384-cp
Subject(s) - ion semiconductor sequencing , in silico , amplicon , context (archaeology) , dna sequencing , computational biology , bioinformatics , biology , medicine , genetics , oncology , gene , polymerase chain reaction , paleontology
In 2016, the College of American Pathologists (CAP) launched the first next-generation sequencing (NGS) in silico bioinformatics proficiency testing survey to evaluate the performance of clinical laboratory bioinformatics pipelines for the detection of oncology-associated variants at varying allele fractions. This survey focused on 2 commonly used oncology panels, the Illumina TruSeq Amplicon Cancer Panel and the Thermo Fisher Ion AmpliSeq Cancer Hotspot v2 Panel.