Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates | Zendy

Keyvan Pakshir | Zendy; Fatemeh Karimi | Zendy; Kamiar Zomorodian | Zendy; Saham Ansari | Zendy; Hasti Nouraei | Zendy; Alireza Gharavi | Zendy

Open Access

Molecular Discrimination of the Candida parapsilosis Species Complex via SADH Gene Analysis and Evaluation of Proteinase Activity Among the Isolates

Author(s) -

Keyvan Pakshir,

Fatemeh Karimi,

Kamiar Zomorodian,

Saham Ansari,

Hasti Nouraei,

Alireza Gharavi

Publication year - 2018

Publication title -

jundishapur journal of microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.281

H-Index - 29

eISSN - 2008-4161

pISSN - 2008-3645

DOI - 10.5812/jjm.69782

Subject(s) - candida parapsilosis , biology , sensu stricto , microbiology and biotechnology , gene , polymerase chain reaction , virulence , genetics , antifungal , zoology

Background: Candida parapsilosis consists of three species of C. parapsilosis sensu stricto, C. metapsilosis, and C. orthopsilosis. Proteinase activity is considered as one of the major virulence factors in the pathogenicity of Candida species. Objectives: We aimed to discriminate clinical isolates of the C. parapsilosis species complex through analysis of the secondary alcohol dehydrogenase (SADH) gene using BanI restriction enzyme and evaluation of proteinase activity among the isolates. Methods: In this study, 71 clinical isolates identified as C. parapsilosis were included. The species were discriminated via analysis of SADH gene using S1F and S1R primers. The polymerase chain reaction (PCR) products were digested with restriction enzyme BanI. Proteinase activity of the species was evaluated using bovine serum albumin agar medium. To analyze the data, Fisher’s exact test

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