Expression of NS3/NS4A Proteins of Hepatitis C Virus in Huh7 Cells Following Engineering Its Eukaryotic Expression Vector
Author(s) -
Mohammad Amin Behzadi,
Abdolvahab Alborzi,
Gholamreza Pouladfar,
Mehdi Dianatpour,
Mazyar Ziyaeyan
Publication year - 2015
Publication title -
jundishapur journal of microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.281
H-Index - 29
eISSN - 2008-4161
pISSN - 2008-3645
DOI - 10.5812/jjm.27355
Subject(s) - expression vector , ns3 , microbiology and biotechnology , lipofectamine , transfection , vector (molecular biology) , immunogenicity , biology , virology , recombinant dna , antibody , hepatitis c virus , virus , gene , biochemistry , genetics
This study can serve as a fundamental experiment for the construction of a NS3/NS4A eukaryotic expression vector and its expression in mammalian cells. Further research is underway to evaluate the fragment immunogenicity in lab animal models.
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