Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage | Zendy

Mohammad Sadegh Hashemzadeh | Zendy; Rahimeh Rasouli | Zendy; Bentolhoda Zahraei | Zendy; Morteza Izadi | Zendy; Mahdi Tat | Zendy; Seyed Hassan Saadat | Zendy; Mohammad Najarasl | Zendy; Behzad Khansari nejad | Zendy; Ruhollah Dorostkar | Zendy

Open Access

Development of Dual TaqMan Based One-Step rRT-PCR Assay Panel for Rapid and Accurate Diagnostic Test of MERS-CoV: A Novel Human Coronavirus, Ahead of Hajj Pilgrimage

Author(s) -

Mohammad Sadegh Hashemzadeh,

Rahimeh Rasouli,

Bentolhoda Zahraei,

Morteza Izadi,

Mahdi Tat,

Seyed Hassan Saadat,

Mohammad Najarasl,

Behzad Khansari nejad,

Ruhollah Dorostkar

Publication year - 2016

Publication title -

iranian red crescent medical journal

Language(s) - English

Resource type - Journals

eISSN - 2074-1812

pISSN - 2074-1804

DOI - 10.5812/ircmj.23874

Subject(s) - taqman , middle east respiratory syndrome coronavirus , virology , rna , polymerase chain reaction , reverse transcription polymerase chain reaction , real time polymerase chain reaction , gene , medicine , computational biology , biology , covid-19 , genetics , messenger rna , disease , infectious disease (medical specialty) , pathology

This study showed that the developed rRT-PCR assays are rapid, reliable, reproducible, specific, sensitive, and simple tools for detection of MERS-CoV. Finally, a kit consisting of two assay signatures and controls was assembled, which can be distributed to public health laboratories in Iran to support international MERS-CoV surveillance and public health response.

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