Anthelmintic Activity of Ethanol Extract of Polygonum minus Leaves against Ascaridia galli

Anthelmintic Activity of Ethanol Extract of Polygonum minus Leaves against Ascaridia galli Dara Agusti Maulidya 1 , Muhammad Ibnu Kahtan 2 , Diana Natalia 2 , Mitra Handini 3 , Ari Widiyantoro 4 1 University of Tanjungpura, Faculty of Medicine, Pontianak, West Kalimantan, Indonesia 2 University of Tanjungpura, Faculty of Medicine, Department of Parasitology, Pontianak, West Kalimantan, Indonesia 3 University of Tanjungpura, Faculty of Medicine, Department of Physiology, Pontianak, West Kalimantan, Indonesia 4 University of Tanjungpura, Faculty of Mathematics and Science, Department of Chemistry, Pontianak, West Kalimantan, Indonesia ABSTRACT Objectives: Helminth infection is one of the main matters that cause health problems in developing countries. Polygonum minus are empirically used as an anthelmintic drug, but there has been no scientific study that proves it. Methods: This study was aimed at determining the anthelmintic activity of ethanol extract of Polygonum minus leaves against Ascaridia galli in vitro. The extract was prepared in three different concentrations i.e 0.5 mg/mL, 1 mg/mL and 2 mg/mL. NaCl 0.9% was used as a negative control group and albendazole 0.2 mg/mL, a broad spectrum anthelmintic was used as a positive control group. Ascaridia galli was used as the test parasite. The study involved the determination of death time of the worms. Results: The death time of the worms that caused by the extract concentration 0.5 mg/mL, 1 mg/mL and 2 mg/mL, respectively were (mean±SD) 35±3.32 hours, 32.8±2.39 hours and 25±3.39 hours. The death time that caused by negative and positive control group were 76.6±8.30 hours and 19.2±1.48 hours. There is no significant difference of death time of the worms between the extract concentration 2 mg/mL and the positive control group. Conclusion: The anthelmintic activity of ethanol extract of Polygonum minus leaves has been confirmed and extract concentration 2 mg/mL has the same anthelmintic activity with albendazole 0.2 mg/mL. J Microbiol Infect Dis 2018; 8(1):23-26 Keywords: Anthelmintic, Polygonum minus , Ascaridia galli , ascariasis